朱作言文集(全二册)

本书特色

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　　朱作言先生研究团队于20 世纪80 年代初期首创农业动物基因工程研究，在1985 年发表了世界**篇鱼类基因转移研究文章，享有世界声誉。《朱作言文集（全2册）》从该团队发表的200 多篇文章中，选取了100 篇编辑成册，分上卷和下卷出版。《朱作言文集（全2册）》的主线是基于转基因鱼理论模型的建立、转基因鲤研制及品系选育、转植基因功能，以及转基因鱼食用和生态安全研究。内容共包括7 个部分：**部分和第二部分建立了转基因鱼研究的完整理论模型，并以此为基础成功研制转“全鱼”生长激素基因黄河鲤家系，系统揭示了转植基因的整合、表达和遗传规律。第三部分从摄食、代谢、生长、免疫和游泳行为等方面，详细描述了转基因黄河鲤与野生黄河鲤的生物学特性。第四部分系统评估了转基因鱼在实验室及模拟自然环境条件下的生态学效应。第五部分系统研究了鱼类生长激素对性腺发育的调控作用、育性控制技术及研制不育转基因鱼的策略。第六部分包括草鱼全基因组草图绘制、草鱼病原病毒分子生物学和抗病草鱼基因工程育种的基础研究。第七部分主要以斑马鱼为模型，对鱼类基因工程的生物学原理、技术拓展和应用进行了全方位的探讨。为了便于广大读者阅读，英文文章后面均附有中文摘要。

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目录

目 录上 卷**部分 鱼类基因转移模型建立Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3人生长激素基因在泥鳅受精卵显微注射转移后的生物学效应 8Introduction of Novel Genes into Fish 11转基因鱼模型的建立 23第二部分 外源基因的整合与表达The β-Actin Gene of Carp (Ctenopharyngodon idella) 35Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from GrassCarp (Ctenopharyngodon idellus) 54Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are HighlyPolymorphic 73Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp(Cyprinus carpio L ) 87Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for theSelection of Site-Directed Knockout/Knockin 101Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre RecombinaseUsing A Combination of Mutant Lox Sites 114Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence(EGS) Technique 128Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation inTransgenic Zebrafish Embryos 137Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia(Oreochromis niloticus) 172Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UASSystems 189Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes inZebrafish Using TALENs 211Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNADouble-Strand Breaks in Zebrafish Embryos 225第三部分 外源基因表达的生理功能Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function inHypophysectomized Carp 249Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinuscarpio L red var ) 263Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic RedCommon Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes ThymusDevelopment 276Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-SpecificImmune Functions of Common Carp， Cyprinus carpio L 287Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: ReducedCritical Swimming Speed 298Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic CommonCarp 307Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinuscarpio L ) 334Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of GrowthHormone (cGH) in Common Carps (Cyprinus carpio) 350The Hematological Response to Exhaustive Exercise in ‘All-Fish’ GrowthHormone Transgenic Common Carp (Cyprinus carpio L ) 362Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth HormoneTransgenic Common Carp (Cyprinus carpio L ) 376Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and BoneDevelopment in Common Carp 386Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401第四部分 转基因黄河鲤的生态安全研究Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and ItsContainment Strategies 423Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpioAssociated with Their Morphological Variations 433Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ GrowthHormone Transgenic Common Carp Cyprinus carpio 446Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )Under Short-Term Predation Risk 462Behavioral Alterations in GH Transgenic Common Carp May Explain EnhancedCompetitive Feeding Ability 471Risk-Taking Behaviour May Explain High Predation Mortality of GH-TransgenicCommon Carp Cyprinus carpio 489Transgenic Common Carp Do Not Have the Ability to Expand Populations 506Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:Implications for Environmental Risk 518第五部分 鱼类生殖调控与转基因鱼的育性控制研究A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546Identification and Characterization of A Novel Splice Variant of Gonadotropin αSubunit in the Common Carp Cyprinus carpio 560Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin

封面

书名:朱作言文集(全二册)

作者:汪亚平[等]主编

页数:2册(20,1404

定价:¥880.0

出版社:科学出版社

出版日期:2019-03-01

ISBN:9787030604613

PDF电子书大小:135MB 高清扫描完整版